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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: Conventional susceptibility tests of a total of 100 Klebsiella pneumoniae clinical isolates and the diagnostic efficiency of the antibiotic resistance determined by the SYBR Green I/PI staining.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: Diagnostic Assay, SYBR Green Assay, Staining
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: Growth of Klebsiella pneumoniae in vitro with growth curve (A) and the representative fluorescence images (400 × magnification) of SYBR Green I/PI staining at 0, 2, 4, 6, 8, and 10 h, respectively (B) .
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: In Vitro, Fluorescence, SYBR Green Assay, Staining
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: The linear regression relationship between the percentage of live bacteria and the S value based on the SYBR Green I/PI staining of K. pneumoniae measured using a fluorescence reader (A) and the representative images of fluorescence microscopy at different proportions (0, 50, 80, and 100%) of live bacteria (B) . The green and red dots represent the live and dead cells of K. pneumoniae , respectively.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: Bacteria, SYBR Green Assay, Staining, Fluorescence, Microscopy
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: Antibiotic effects revealed by SYBR Green I/PI staining on both sensitive and resistant strains of K. pneumoniae treated with ceftriaxone (A) , cefepime (B) , meropenem (C) , and ciprofloxacin (D) of incremental concentrations for 40 min. The symbols of red dots and blue triangles represent the resistant and sensitive strains of bacteria, respectively. Statistical differences set at p < 0.05, p < 0.005, and p < 0.0005 are indicated by symbols *, **, and ***, respectively.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: SYBR Green Assay, Staining, Bacteria
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: Representative images (630 × magnification) of SYBR Green I/PI staining of the sensitive (A) and resistant (B) clinical isolates of K. pneumoniae at their stationary phases treated with four types of antibiotics (i.e., ceftriaxone, cefepime, meropenem, and ciprofloxacin). The control groups contain no antibiotics. The green and red dots indicate the live and dead bacteria, respectively.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: SYBR Green Assay, Staining, Control, Bacteria
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: Antibiotic resistance of a total of 100 clinical isolates to ceftriaxone (A) , cefepime (B) , meropenem (C) , and ciprofloxacin (D) evaluated using the SYBR Green I/PI staining. The horizontal dashed lines (---) indicate the cutoff values of the resistance determination based on the SYBR Green I/PI staining. Strains identified as resistant (R) and sensitive (S) clinical strains identified in the conventional susceptibility tests are shown in red dots and blue triangles, respectively. The symbol “***” indicates significant differences set at p < 0.0005.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: SYBR Green Assay, Staining
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: The receiver operating characteristic (ROC) curve based on the antibiotic susceptibility to ceftriaxone (A) , cefepime (B) , meropenem (C) , and ciprofloxacin (D) of a total of 100 clinical isolates detected by SYBR Green I/PI staining. AUC is the area under the ROC curve.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: SYBR Green Assay, Staining
Journal: Frontiers in Microbiology
Article Title: Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
doi: 10.3389/fmicb.2021.650458
Figure Lengend Snippet: Retesting of inconsistent strains by the conventional AST and the SYBR Green I/PI staining.
Article Snippet: The results showed a high degree of reliability and validity of the
Techniques: SYBR Green Assay, Staining